We wanted a way of searching for amino acid substitutions across orthologs so we could find versions that replaced a cysteine residue for something else, and see the amino acid context of that change so we could engineer a hybrid protein with the substitution in its proper context. We broadened this so one could use it to see if, say, a disease variant is tolerated in an ortholog, and then see the discrete 7-residue context it sits in. The result is the Ortholog Residue Subst

DEEPN (Dynamic Enrichment for Evaluation of Protein Networks) is our lab's fully integrated suite of processing and analysis programs for batch yeast two-hybrid (Y2H) screens: from fastq to prey fragment discovery. It maps raw sequencing reads (MAPster), counts them per candidate gene, extracts junction sequences, and statistically ranks candidate protein-protein interactions. Stat Maker is the statistics module at its core. Version 7 adds a more rigorous statistics panel for

K48-ubiquitin-dependent proteases cut-up post-ER proteins Authors: Annabel Y Minard, Stanley Winistorfer, Liping Yu, Robert C Piper 1. Department of Molecular Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, IA 52242, United States. 2. Biomolecular Nuclear Magnetic Resonance Facility, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA. bioRxiv 2025.04.13.648637; doi: https://doi.org/10.1101/2025.04.13.648637 In Press-- Nature

Insulated piggyBac and FRT vectors for engineering transgenic homozygous and heterozygous eHAP cells. Minard AY*, Winistorfer S, Piper RC. Biol Open. 2025 Oct 15;14(10):bio061793. doi: 10.1242/bio.061793. Epub 2025 Oct 1. PMID: 40856007 * corresponding Annabel and Stan made a series of expression vectors that confers durable expression in a number of cell types, including eHAP haploid cells. This works in part due to the incorporation of insulator sequences around the express

Charcot-Marie-Tooth disease type 1E: clinical natural history and molecular impact of PMP22 variants Kailee S Ward , Christopher P Ptak , Natalya Pashkova , Tiffany Grider , Tabitha A Peterson , Davide Pareyson , Chiara Pisciotta , Paola Saveri , Isabella Moroni , Matilde Laura , Joshua Burns , Manoj P Menezes , Kayla Cornett , Richard Finkel , Bipasha Mukherjee-Clavin , Charlotte J Sumner , Maxwell Greene , Omer Abdul Hamid , David Herrmann , Reza Sadjadi , David Walk , Step

This study unveils the dynamic partnership between two key myelin proteins, PMP22 and MPZ, which are both critical for healthy peripheral...

Led by Chris Ptak, structural studies reveal subregions on MPZ that correlate with disease phenotypes. These data also MPZ works in yet...

The electrophysiological properties of NaV channels are routinely determined in heterologous systems, such as human embryonic kidney...